CRISPR Toolset

Click here to identify guide RNA target sites in a user specified yeast gene

Click here to identify guide RNA target sites in a user specified yeast chromosomal region

Click here for designing oligos for cloning a user-designed guide RNA sequences

Links to websites about CRISPR-Cas9:
AddGene CRISPR Informational Page
NEB CRISPR Informational Page

Example mutagenic oligonucleotide template design: